Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66.9kD protein (594aa) selectively expressed in gabanergic neurons of the CNS.
It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter b-amino butyric acid (GABA)
An affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsWestern Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Glutamic Acid Decarboxylase (GAD-67). Species Crossreactivity: mouse.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to a region near the C- terminus of Glutamic Acid Decarboxylase (GAD-67) (KLH). Species sequence homology: human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) 100%.
