Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).
Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906:IgY, Chicken Pab Rb x Ch I1906-10:IgY, Chicken (HRP) Rb x Ch I1906-12:IgY, Chicken (FITC) Rb x Ch I1906-14:IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:chicken
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2, 0.075% sodium azide