Keyhole Limpet Hemocyanin (KLH) is generally a very efficient coupling agent.
It is the most common carrier protein used in the preparation of hapten conjugates.
KLH is composed of five subunits.
It is lysine rich with a large number of available primary amines to facilitate conjugation, antibody production and promote peptide attachment after dissociation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk x ChI1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk x ChI1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk x ChI1906: IgY Pab Rb x ChI1906-01: IgY, Fc (HRP) Pab Gt x ChI1906-10: IgY (HRP) Pab Rb x ChI1906-12: IgY (FITC) Pab Rb x ChI1906-14: IgY (AP) Pab Rb x Ch
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：chicken
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 20% glycerol. Labeled with Horseradish peroxidase (HRP).
