TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity.
Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation.
ERK activation was enhanced by TRIB1.
Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1.
Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human TRIB1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human TRIB1 (KLH).
