TP53INP2/DOR is a scaffold protein that recruits LC3 and/or LC3-related proteins to the autophagosome membrane by interacting with the transmembrane protein VMP1.
TP53INP2 translocates from the nucleus to the autophagosome structures after activation of autophagy by rapamycin or starvation.
TP53INP2 is necessary for autophagosome development because its small interfering RNA-mediated knockdown strongly decreases sensitivity of mammalian cells to autophagy.
Applications:Suitable for use in ELISA, Indirect Flow Cytometry, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Indirect Flow Cytometry: 1:10-1:50Immunohistochemistry: 1:50-1:100Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human TP53INP2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH conjugated synthetic peptide between corresponding to a portion of amino acids within aa 179~208 in the C-terminal region of human TP53INP2
