Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals.
The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle.
Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human PDK1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the N-terminal region of human PDK1 (KLH).
![Pyruvate Dehydrogenase Kinase Isoform 1, NT (Pyruvate Dehydrogenase Kinase Isozyme 1, [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 1 Mitochondrial, PDK1) 200ul](https://aimg.as-1.co.jp/c/84/1158/32/85133520.jpg)