cGKII is thought to play a key role in a diverse set of physiological pathway.
cGKII may mediate intestinal secretion of water and electrolytes induced by the E.
coli toxin STa and the intestinal peptide guanylin.
Edentification of the pathway that mediates intestinal fluid secretion by E.
coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries.
Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry.
Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference.
cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human cGKII.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human cGKII (KLH).
