Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.
PKC also serve as major receptors for phorbol esters, a class of tumor promoters.
Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells.
PKC nu is one of the PKC family members.
This kinase can be activated rapidly by the agonists of G protein-coupled receptors.
It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation.
This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications have not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human PKC nu.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 860-890 in the C-terminal region of human PKC nu. Species sequence homology: mouse
