MTMR, a member of the myotubularin family, encodes an intracellular protein of 1,195 amino acids, and shares approximately 47% sequence identity with MTMR3, spiking to over 70% identity in the catalytic domain and the double zinc finger (FYVE) motif.
PCR analysis detects expression of MTMR4 in all human tissues examined except lung, small intestine, stomach, salivary gland, adrenal gland, and uterus.
Subcellular localization of MTMR4 in transfected HeLa cells showed a perinuclear distribution, similar to that seen with MTMR3.
MTMR4 dephosphorylates para-nitrophenylphosphate and phosphatidylinositol 3-phosphate.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human Myotubularin Related Protein 4. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1155-1187 in the C-terminal region of human Myotubularin Related Protein 4.
