Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR).
LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product.
During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases.
The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.
Applications:Suitable for use in ELISA, Immunohistochemistry, Western Blot and Indirect Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human Leptin Receptor.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 23-52 in the N-terminal region of human Leptin Receptor.
