Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets.
It is the major enzyme yielding glutamate from glutamine.
Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981).
High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:2000Immunohistochemistry (FFPE, Leica): 1:500Immunofluorescence: 1:25Flow Cytometry: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human Glutaminase, Kidney Isoform, Mitochondrial. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within aa516-545 in the C-terminal region of human Glutaminase, Kidney Isoform, Mitochondrial. Species Sequence Homology: rat.
