84-1149-81　F10, CT (Coagulation Factor X, Stuart Factor, Stuart-Prower Factor, Factor X Light Chain, Factor X Heavy Chain, Activated Factor Xa Heavy Chain) 200ul　F0019-40

特徴

• F10 is the vitamin K-dependent coagulation factor X of the blood coagulation cascade.
• This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR.
• Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases.
• The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway).
• The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting.
• Applications:Suitable for use in Immunohistochemistry, Indirect Flow Cytometry, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Indirect Flow Cytometry: 1:10-1:50Immunohistochemistry (Paraffin): 1:50-1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.