EN2 is thought to have a role in controlling development.
In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments.
Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal.
This protein has been implicated in the control of pattern formation during development of the central nervous system.
Applications:Suitable for use in Indirect Flow Cytometry, Immunohistochemistry, ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography.
Form:Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:Recognizes human EN2. Species Crossreactivity: mouse
Isotype:IgG
Calc Applications Abbrev:E FC IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 243-271 in the C-terminal region of human EN2.