Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment.
These molecules are involved in the regulation of cell growth, differentiation and metabolism.
MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen.
This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain.
Its autophosphorylation is achieved by all collagens so far tested (type I to type VI).
In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain.
In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain.
The gene is located on chromosome 6p21.3 in proximity to several HLA class I genes.
Applications:Suitable for use in ELISA, Western Blot, Indirect Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (FFPE): 1:10-1:50Indirect Flow Cytometry: 1:125Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human DDR1. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 17-47 in the N-terminal region of human DDR1, Swiss/UniProt Accession: Q08345; Q63474; Q03146. Species Sequence Homology: mouse
