The adenylate kinases (AK) are a family of structurally and functionally related enzymes that catalyze a similar reaction, MgNTP + AMP = MgNDP + ADP (N = A or G).
The AK enzymes are important for maintenance of homeostasis of the adenine and guanine nucleotide pools.
AK1 is a cytosolic enzyme for which ATP is the substrate.
AK2 catalyzes the same reaction as AK1, but it is localized in the mitochondrial intermembrane space.
AK3 is present in the mitochondrial matrix and prefers GTP over ATP as the substrate.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:Recognizes human AK7.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide selected from the C-terminal region of human AK7 (KLH).