PCNA is found in the nucleus and is a cofactor of DNA polymerase delta.
This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.
In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:10-1:50Western Blot: 1:1000Immunohistochemistry (paraffin): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human PCNA.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within 231-261 amino acids from the C-terminal region of human PCNA. Uniprot/Swiss Accession: P12004. Species sequence homology: bovine, hamster, monkey and rat
