MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue.
Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread.
It could be an adhesion molecule active in neural crest cells during embryonic development.
It acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (FFPE): 1:50-1:100Western Blot: 1:50-1:100Indirect Flow Cytometry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human MCAM at ~71kD.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa168-197 from the center region of human MCAM, conjugated to KLH. Cellular Localization: Membrane; SIngle-pass type 1 membrane protein
