Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements.
The subunits display a tissue-specific pattern of expression.
Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Immunohistochemistry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:Recognizes human Vimentin.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide selected from the center region of human Vimentin (KLH).