The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M.
Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus.
The full-length cDNA encodes a 747aa protein with a putative ATP/GTP-binding site motif.
RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues.
Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney.
P100 is also correlated to cancer prognosis.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human P100.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：A mixture of 3 KLH-conjugated synthetic peptides selected from C-terminal region with phenylalanine at position 624, C-terminal region with serine at position 729, and center region of human NRG4 (KLH).
