SIGLEC9 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells.
It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
This protein is expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils).
It is found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.
SIGLEC9 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM).
This motif is involved in modulation of cellular responses.
The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human SIGLEC9. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the C-terminal region of human SIGLEC9 (KLH).
