SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells.
It preferentially binds to alpha2,3-linked sialic acid.
and also binds to alpha2,6-linked sialic acid.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
SIGLEC8 is expressed specifically on eosinophils.
The protein contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM).
This motif is involved in downmodulation of cellular responses.
The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1.
The SIGLEC8 gene belongs to the immunoglobulin superfamily.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human SIGLEC8. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 373-406 in the C-terminal region of human SIGLEC8, UniProt Accession #Q9NYZ4.
