LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors.
LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors.
Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk.
Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified from rabbit antisera
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human LIME.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Bacterially expressed intracellular fragment corresponding to aa141-295 of human LIME.
