84-1132-25　［取扱停止］Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, AHUS5, ARMD9, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, Complement Component 3, Complement Factor 3, CPAMD1) (FITC) 1ml　C7850-14R

特徴

• C3 consists of two polypeptide chains (alpha, MW: 115kD and beta, MW: 75kD) and is usually present in serum at 1 to 2 mg/L.
• It is the pivotal protein of the complement system, being central in the classical pathway and the alternative pathway.
• It is cleaved by the classical pathway convertase C4b2a and the alternative pathway convertase C3bBb, into C3b and C3a.
• The C3a peptide is an important anaphylotoxin, whilst the C3b has three main roles: 1.
• Important opsonin phagocyte cells have C3b receptors.
• 2.
• Participates in the amplification loop in the alternative pathway.
• 3.
• Complexed with C4b2a this is the C5 convertase which leads ultimately to target cell lysis.
• C3c, along with iC3b and C3dg, is formed as a result of C3b inactivation by Factor I and various cofactors.
• The C3 fragments are eliminated by liver phagocytic cells.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Frozen): 1:50-1:100 using the Direct Method
• Optimal dilutions to be determined by the researcher.
• Positive Control:Human skin
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.