84-1132-21　［取扱停止］Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3, COL4A3, Collagen alpha-4(IV) Chain, Collagen Type IV Alpha 4, COL4A4, Collagen alpha-5(IV) Chain, Collagen Type IV Alpha 5, COL4A5, Collage ...　C7510-53M

特徴

• Human plasma proteins do not interfere with collagen binding.
• Collagen is located in the extracellular matrix of connective tissues.
• It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
• Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina.
• Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used.
• If FITC is used dilute 1:10.
• Not tested on paraffin
• Immunocytochemistry: Cultured human cellsELISA: Native human collagen IVDot Blot: Native human collagen IVOptimal dilutions to be determined by the researcher.
• IHC Positive Control:Human kidney, liver, skin, heart
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with 1ml ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 6 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.