84-1132-19 ［取扱停止］Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3, COL4A3, Collagen alpha-4(IV) Chain, Collagen Type IV Alpha 4, COL4A4, Collagen alpha-5(IV) Chain, Collagen Type IV Alpha 5, COL4A5, Collage ... C7510-53J
84-1132-19 ［取扱停止］Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3, COL4A3, Collagen alpha-4(IV) Chain, Collagen Type IV Alpha 4, COL4A4, Collagen alpha-5(IV) Chain, Collagen Type IV Alpha 5, COL4A5, Collage ... C7510-53J
Human plasma proteins do not interfere with collagen binding.
Collagen is located in the extracellular matrix of connective tissues.
It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina.
Applications:Suitable for use in Immunohistochemistry, ELISA and Dot Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:20 if peroxidase labeled secondary is used.
If FITC is used, dilute 1:10.
Not tested on paraffin
ELISA: Native human Collagen IVDot Blot: Native human Collagen IVOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by ammonium sulfate precipitation and DEAE chromatography
Form：Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes human Collagen IV. Minimal crossreactions with other collagens were only found if 5-10 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin.
Isotype：IgG
Calc Applications Abbrev：DB E IHC
Calc Crossreactivity：Hu
Immunogen：Native human Collagen IV
Shelf Life：1year
EU Commodity Code：30021010
商品名：Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3, COL4A3, Collagen alpha-4(IV) Chain, Collagen Type IV Alpha 4, COL4A4, Collagen alpha-5(IV) Chain, Collagen Type IV Alpha 5, COL4A5, Collagen alpha-6(IV) Chain, Collagen Type IV Alpha 6, COL4A6, Arresten, ASLN, ATS, CA44, CA54, Canstatin, DKFZp686I14213, FLJ22259, Goodpasture Antigen, MGC42377, MGC167109) 1ml
