CD54 (ICAM-1) permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactions or for rhinovirus and malarial studies.
The LFA-1 binding site is located in domain 1 of ICAM-1.
CD54 is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system.
CD54 is weakly expressed on resting peripheral blood lymphocytes.
Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.
ICAM-1 has 7 potential N-linked glycosylation sites.
ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes.
ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1).
This interaction enhances antigen-specific T-cell activation.
ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs.
Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1.
CD54 is the receptor for rhinoviruses and malaria.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:10Immunohistochemistry (Frozen): 1:10Note: For selection of the most useful dilution, a test staining with cells or tissue know to express the antigen should be performed.
A small fresh skin biopsy can be incubated for 4 hours with TNF-a (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37°C
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
Specificity：Recognizes both intact human CD54, ICAM-1 and soluble human ICAM-1. Species Crossreactivity: chimpanzee, Rhesus monkey, Cynomolgous monkey, baboon tissues.
