Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C.
elegans cell death gene (ced-3).
Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms.
This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-1ug/ml; Detects a cleaved subunit band of ~21kD in human heart cell lysates (predicted precursor MW 45.2kD).
Immunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g.
trypsin or pronase prior to staining.
Optimal dilutions to be determined by the researcher.
IHC Positive Control:Human heart tissue.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes an epitope within the C-terminal region (CT) of human Caspase-1.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：16 amino acid peptide located near human Caspase-1 carboxy terminus.
