The apolipoproteins are family of amphipathic lipoproteins that plays a crucial role in lipid metabolism.
In particular, apolipoprotein E (apo E) is a major component of high density lipoproteins (HDL) chylomicrons and very low-density lipoprotein (VLDL), which functions to remove excess cholesterol.
Apo E mediates lipid metabolism and cholesterol homeostasis by binding to several hepatic lipoprotein receptors, such as low density lipoprotein receptor (LDLR) and the LDLR-related protein.
Apo E deficiency is linked to hyperlipoproteinemia type III; a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease.
Three major isoforms of Apo E (E2, E3, E4) have been identified, with E3 being the most common in human.
E4 presence has been associated to increase probability of developing late-onset form of Alzheimer disease.
Applications:Suitable for use in ELISA, Dot Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000 on native and denatured human apoE
Dot Blot: Native and denatured human apoE
Immunohistochemistry (Frozen): 1:30 Immunocytochemistry: Cultured human cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. The buffer composition prior to lyophilization is 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol.
Specificity：Recognizes human Apolipoprotein E. Specificity is confirmed by direct ELISA with purified human ApoE adsorbed to plastic wells, by Ouchterlony immunodiffusion, and by dot-blot assay with different fractions of lipoproteins.
