84-1131-93　［取扱停止］Apolipoprotein A-I (Apolipoprotein A-I Precursor, Apolipoprotein AI, Apo AI, Apo A-I, ApoA-I, Apo-AI, Apolipoprotein A1, APOA1, Ltw-1, Lvtw-1) 500ug　A2299-12M

特徴

• Apolipoprotein AI promotes cholesterol efflux from tissues to the liver for excretion.
• Apolipoprotein AI is the major protein component of high density lipoprotein (HDL) in the plasma.
• Synthesized in the liver and small intestine, it consists of two identical chains of 77aa; an 18aa signal peptide is removed co-translationally and a 6aa propeptide is cleaved post-translationally.
• Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters.
• Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease.
• The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I.
• The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
• Applications:Suitable for use in ELISA, Dot Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000; use on native and denatured human ApoA1
• Dot Blot: Use on native and denatured human ApoA1
• Immunohistochemistry (Frozen): 1:40 Immunocytochemistry: Cultured human cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.