Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2).
Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4) and Piwi-interacting RNAs (piRNAs) (5), have been identified.
MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation and stress response (1,2).
MicroRNAs regulate gene expression by modulating mRNA translation or stability (2).
MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2).
Among the most important components in these complexes are Argonaute proteins (1,2).
There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2).
Argonaute proteins participate in the various steps of microRNA-mediated gene silencing such as repression of translation and mRNA turnover (1).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human eIF2C1 at ~97kD. Species Crossreactivity: Mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Lys400 of human eIF2C1.
