Acinus (apoptotic chromatin condensation inducer in the nucleus) is a caspase substrate that has been implicated in nuclear changes during apoptosis (1).
Chromatin condensation and DNA fragmentation are both nuclear morphological features associated with apoptosis.
Acinus is expressed in different isoforms (L, S, S') most likely generated by alternative splicing (1).
During apoptosis Acinus is cleaved by caspase-3 to generate a 23kD fragment that was reported to induce chromatin condensation (1).
Acinus has been identified to be a component of the spliceosome complex, ASAP, suggesting a role in pre-mRNA processing (2-4).
Down regulation of Acinus by RNA interference inhibits cell growth (5).
This study also found that loss of Acinus inhibits DNA fragmentation but not chromatin condensation during apoptosis.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence (IF-IC): 1:50Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Acinus protein. Recognizes L, S and S' isoforms. Species Crossreactivity: monkey, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Ala835 of Acinus.
