The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-kB (2-4).
Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).
ABIN-1 (Naf1/TNIP1) was identified based on its binding to A20 (6), as well as the HIV protein Nef-1 (7).
Overexpression of ABIN-1 inhibits NF-kB activation by a number of stimuli including TNF, IL-1, and LPS.
It is widely expressed and can be induced by NF-kB, providing a negative feedback loop of NF-kB signaling.
In addition to binding to A20, ABIN-1 can function on other key components of NF-kB signaling including IKKgamma/NEMO (8) and NF-kB family members p100 and p105 (9), and can also inhibit ERK signaling (10).
Knockout mice of ABIN-1 are embryonic lethal, with fetal liver apoptosis, anemia and hypoplasia (11).
ABIN-1 deficient cells are hypersensitive to TNF-mediated apoptosis.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight
mmunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human total ABIN-1 protein. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues near the N-terminus of human ABIN-1. Species sequence homology: monkey (100%)
