84-1131-68　14-3-3 eta (14-3-3 Protein eta, Protein AS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein eta Polypeptide, Tyrosine 3/tryptophan 5-monooxygenase Activation Protein eta Polypeptide, YWHA1, YWHAH, HGNC:12853) 100ul　0014-06A

特徴

• The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways.
• 14-3-3 proteins are highly conserved and ubiquitously expressed.
• There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals.
• The initially described alpha and delta isoforms are confirmed to be phosphorylated forms of beta and zeta respectively.
• Through their amino-terminal alpha helical region, 14-3-3 proteins forms homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases and other signaling molecules.
• The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif.
• However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed.
• 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability and molecular interactions.
• 14-3-3 proteins may also induce target protein conformational changes which modify target protein function.
• Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in develop- ment and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform differ- ent functions despite their sequence similarities.
• Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000.
• Incubate membranes with diluted antibody, 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking overnight
• Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap