ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity.
PATHWAY: Catecholamine biosynthesis; first step.
SUBUNIT: Homotetramer.
PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.
SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia.
Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1-2000-1:5000Western Blot: 1:100-1:500 on RIPA-extracted PC12 cell lysatesOptimal dilutions to be determined by the researcher.
Positive Control:Mouse or rat brain containing catecholaminergic neurons (brain stem or striatum).
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes Hydroxylase (TH). Species Crossreactivity: rat, gunea pig and mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Gp Mo Rt
Immunogen：Synthetic peptide corresponding to aa63-78, PRFIGRRQSLIEDARK from human Tyrosine Hydroxylase, conjugated to KLH. Species Sequence Homology: rat and mouse, aa32-47
