Tumor necrosis factor receptor 1 (TNF Receptor 1) binds cytokines TNF-alpha and TNF-beta resulting in the activation of pathways involved in both cell survival and apoptosis.
TNF Receptor 1 contains an extracellular death domain that binds to the death domain adaptor protein TRADD and triggers the activation of caspases.
At least 3 isoforms are produced by alternative splicing.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 0.5-1ug/ml using formalin-fixed, paraffin-embedded tissue.
Requires het mediated antigen retrieval
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
Specificity：Recognizes human Tumor necrosis factor receptor 1 at 51kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Gt Hu Rt
Immunogen：Synthetic peptide corresponding to aa120-136, DRDTVCGCRKNQYRHYW to human Tumor necrosis factor receptor 1 (precursor).
