Upon binding of acetylcholine, the acetylcholine receptor responds by undergoing an extensive change of conformation which affects its alpha and beta subunits and leads to the opening of an ion-conducting channel across the plasma membrane.
At least 2 isoforms of the alpha-1 subunit are produced from alternate splicing.
Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.75ug/ml.
Band expected at ~55kD
Immunohistochemistry (FFPE): 1-2ug/ml.
Heat mediated antigen retrieval is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH20.
Specificity：Recognizes human Nicotinic Acetylcholine Receptor alpha 1. Species Crossreactivity: rat. Species Sequence Homology: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa22-36, EHETRLVAKLFKDYS from the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1.
