84-1131-29　［取扱停止］Myelin Basic Protein (MBP, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, MGC99675) 100ug　M9758-01P

特徴

• Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target.
• Myelin isolated from human and bovine nervous tissue is composed of ~80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP).
• MBP is an 'intrinsically unstructured' protein with a high proportion (~75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix.
• MBP is a major protein in CNS myelin and is expressed specifically in the nervous system.
• A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational.
• Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic.
• The message for myelin basic protein is selectively translocated to the ends of the cell processes.
• Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model.
• Applications: Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry (Formalin fixed, paraffin): 1-2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.