The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases.
All MMPs are synthesized as inactive proenzymes.
The activation of these proenzymes is a critical step that leads to degradation of extracellular matrix components such as fibronectin and collagen type III.
At least 2 isoforms of MMP16 are produced by alternate splicing.
The Long isoform is a single-pass type 1 membrane protein.
The Short isoform is secreted.
Both forms of MMP16 activate MMP2 (progelatinase A) by cleavage.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
Specificity：Recognizes human Matrix metalloproteinase-16 at 70kD. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa582-598, YTVFQFKRKGTPRHILY to human Matrix metalloproteinase-16.
