The Insulin receptor binds insulin and has a tyrosine-protein kinase activity.
The receptor is a tetramer of 2 alpha and 2 beta subunits linked by disulfide bonds.
The alpha subunit (135 kDa) is extracellular and contributes to the formation of the ligand-binding domain whereas the beta subunit (95 kDa) contains the kinase domain and a transmembrane domain.
At least two isoforms of the Insulin receptor are produced by alternative splicing.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months afterr receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Insulin receptor subunit alpha at ~156kD. Species Crossreactivity: canine and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Hu Rt
Immunogen：Synthetic peptide corresponding to aa59-75, HLQILLMFKTRPEDFRD from human Insulin receptor (long isoform), conjugated to BSA. Species Sequence Homology: mouse.
