The Histamine H3 receptor is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family.
This protein is primarily expressed in the CNS.
At least 7 isoforms are produced by alternative splicing.
Expression of these isoforms varies with isoform 3 and isoform 7 being highly expressed in the thalamus, caudate nucleus and cerebellum while isoforms 5 and 6 show a poor expression.
Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus.
Isoform 7 is not found in hypothalamus or substantia nigra.
Applications: Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.75ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by imunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile dH2O.
Specificity：Recognizes human Histamine H3 receptor at 49kD. Species Crossreactivity: rat. Sequence Homology: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptid corresponding to aa428-445, CPQKLKIQPHSSLEHCWK to the C-terminus of human Histamine H3 receptor.
