84-1131-08　［取扱停止］Dopamine Beta Hydroxylase, Native (Dopamine beta-hydroxylase, DBH, Dopamine beta Monooxygenase, DBM) 100ul　D9010-03B

特徴

• DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family.
• DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers.
• It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine.
• It binds 2 copper ions and 1 PQQ per subunit .
• Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.
• Applications: Suitable for use in Western Blot, Immunohistochemistry, radioimmunoassay and inhibition of enzymatic activity..
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-500-1:2000Immunohistochemistry: 1-500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.