The enzyme deoxyuridine triphosphate nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP, the immediate precursor of thymidine nucleotides.
At least 3 isoforms are produced by alternative splicing.
Higher mass isoform DUT-M is primarily located in the mitochondria whereas a lower mass isoform DUT-N is found in the nucleus.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1.0ug/mlImmunohistochemistry (Paraffin and Formalin): 1.0-2.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile dH20.
Specificity：Recognizes human dUTP pyrophosphatase. Species Crossreactivity: rat, mouse, rabbit.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rb Rt
Immunogen：Synthetic peptide corresponding to aa136-150, YDLYSAYDYTIPPM to human dUTP pyrophosphatase.
