Cullin-4B is a core component of E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of specific proteins in the cell.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with 1ml sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 1ml sterile dH2O.
Specificity：Recognizes human Cullin-4B. Species Crossreactivity: rat, zebrafish.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt Ze
Immunogen：Synthetic peptide corresponding to aa877-895, DRDYMERDKENPNQYNYIA to the C-terminus of human Cullin-4B.
