Checkpoint homolog CHK2 is an important cell cycle checkpoint regulator.
The protein is rapidly activated by phosphorylation in response to DNA damage and to replication block.
When activated, CHK2 is known to prevent entry into mitosis and has been shown to regulate the tumor suppressor protein p53, leading to cell cycle arrest in G1.
At least 12 isoforms are produced by alternative splicing.
Applications: Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.75ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
Specificity：Recognizes human Checkpoint homolog CHK2 at 61kD. Species Crossreactivity: rat. Sequence Homology: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa427-441, FSEHRTQVSLKDQIT to human Checkpoint homolog CHK2.
