FAS is a single-pass type I membrane protein and a member of the TNF-receptor superfamily.
The interaction between this receptor and the FAS ligand (TNFSF6/FASLG) forms a death-inducing signalling complex that includes Fas-associated death domain protein (FADD), caspase 8 and caspase 10.
Processing of the caspases in this complex leads to apoptosis.
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Formalin fixed, paraffin, formalin/acetone fixed frozen): 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile ddH20.
Specificity：Recognizes rat CD95 at ~37kD. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to aa87-109, YTDRKHYSDKCRRCAFCDEGHGL of rat CD95 (BSA).
