UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates transepithelial urea transport and is a target for regulation by vasopressin.
Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts.
(The antibody also recognizes a second protein from the UT-A gene drivenby transcription from an alternative promotor and expressed in the thin descending limb of Henle, viz.
UT-A2.).
Applications:Suitable for use in Western Blot and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
1ug/ml detected UT-A1 in 20ug of rat kidney tissue lysate by colorimetric immunoblot analysis using IgG goat x rabbit (HRP) as the secondary antibody
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat UT-A1 at ~97 and 127kD. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to aa911-929 at the C-terminal tail of rat UT-A1 (NP_062220).
