84-1130-76　Superoxide Dismutase, Mn (Manganese Superoxide Dismutase, Manganese SOD, Mn SOD, MnSOD, IPO-B, Superoxide Dismutase [Mn] Mitochondrial Precursor, Superoxide Dismutase 2 Mitochondrial, SOD 2, SOD2, SOD-2) 100ul　S8060-25K

特徴

• Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3).
• It works by catalyzing the dismutation of the superoxide radical O2 ̄ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5).
• In general, SODs play a major role in antioxidant defense mechanisms (4).
• There are two main types of SOD in mammalian cells.
• One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm.
• The two subunits of 16kD each are linked by two cysteines forming an intra-subunit disulphide bridge (3).
• The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix.
• It is a homotetramer of 80kD.
• The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30kD and it exists only in the extracellular space(8).
• SOD3 can also be distinguished by its heparin-binding capacity (1).
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Positive Control:0.2ug/ml was sufficient for detection of Mn SOD in 20μg of rat brain tissue extract.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.