The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions.
It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in transepithelial Na+-ion transport (1).
Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2).
ENaCs are composed of three structurally related subunits that form a tetrameric channel, a, B, and y.
The expression of its alpha and beta subunits is enhanced as keratinocytesdifferentiate (3, 4).
The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5).
And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 50% glycerol.
Specificity：Recognizes rat Scnn1a. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide mapping to aa46-68 of the N-terminal tail of rat Scnn1a (NP_113736).
