84-1130-38　［受注停止］Heat Shock Protein 90 (hsp90) 100ul　H1831-58K

特徴

• Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells.
• From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex.
• Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1-2% of cytosolic protein).
• It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins.
• Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling.
• The number of proteins now know to interact with Hsp90 is about 100.
• Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5.
• When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
• In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it.
• In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo.
• Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot:1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.