In most eukaryotes hsp70 genes exist as part of a multigene family.
They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria.
The genes show a high degree of conservation, having at least 5O% identity.
The N-terminal two thirds of hsp70s are more conserved than the C-terminal third.
Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides.
When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kD which lacked peptide binding capacity.
Polypeptide binding ability therefore resided within the C-terminal half.
The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins.
When cells are subjected to metabolic stress (e.g., heat shock) a member of the hsp 70 family, hsp 70 (hsp72), is expressed; hsp 70 is highly related to hsc70 (>90% sequence identity).
Constitutively expressed hsc70 rapidly forms a stable complex with the highly inducible hsp70 in cells following heat shock.
The interaction of hsc70 with hsp 70 is regulated by ATP.
These two heat shock proteins move together in the cell experiencing stress.
Furthermore, research on hsc70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock.
Applications:Suitable for use in Western Blot .
Other applications not tested.
Recommended Dilutions:Western Blot:1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2x50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4 Reconstitute with 50ul or 100ul sterile dH2O.
Specificity：Recognizes hsp70. Species Crossreactivity: Antibodies will recognize Hsp70 proteins (hsc70 and hsp70) from the following samples: fish, Xylella fastidosa-9a5c strain mammalian and plant (tested on A. thaliana, L. sativa, and R. sativa)
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Synthetic peptide conserved in all known Hsp70 and Hsc70 proteins, conjugated to KLH
